Many institutions of higher education (IHEs) that prepare teachers encounter the challenge of increasing requirements of general education preservice teachers so they are properly prepared to teach all students, including those with disabilities. This study examined the impact of a Web-based resource on preservice general education teachers' knowledge regarding assistive technology in the general education classroom. A total of 99 participants enrolled in general education content courses participated in the study. Five different conditions were assessed involving various aspects of a Web-based resource as compared to traditional lecture. Results indicate that required use of a Web-based resource with a graded assignment produced the same results as traditional lecture. However, the Web-based resource as a stand-alone program was not an effective means for increasing preservice teacher knowledge of assistive technology. A Web-based resource could potentially be an efficient and effective way under specific conditions to prepare preservice teachers for diverse classrooms in the 21st century. (Contains 2 tables.)