Usability testing has become a routine way for many libraries to ensure that their Web presence is user-friendly and accessible. At the same time, popular subject guide creation systems, such as LibGuides, decentralize Web content creation and put authorship into the hands of librarians who may not be trained in user-centered design principles. At Metropolitan State University, researchers performed usability testing on LibGuides in order to see how patrons interact with guides. Researchers completed two rounds of usability testing on a total of ten students, one alumnus, and one community patron. The results indicate that patrons struggled most when encountering jargon, inconsistent language, and visual clutter. Based on their findings, researchers recommend ongoing usability testing of subject guides as well as the creation of a style guide to help librarians create usable, accessible guides. (Contains 1 table and 2 figures.)