Background: Development of cultural competence in future health educators is often mentioned as a goal of health education preparation programs; however research demonstrating evidence-based methods for development of cultural competence is limited. Purpose: To determine the impact of a service-learning project on development of cultural competence and health education skills among health education students. Methods: All students in the community health courses completed the Cultural Competence Assessment (CCA) survey at the beginning of the courses; then, 17 students participated in a three-week service-learning project with members of a low-income community. Students repeated the CCA at the end of the course and CCA scores were compared to measure the impact of the service-learning project; additionally, students completed weekly reflective narratives regarding their experiences. Results: Analysis of data indicated that students participating in the service-learning project scored significantly higher on the cultural competence behavior post-test than those that did not; there was a strong association between service-learning participation and cultural competence behavior; and students had increased perceived self-efficacy and cultural competence. Discussion: Service-learning activities can improve health education students' cultural competence. Translation to Health Education Practice: Students completing health education preparation programs having developed cultural competence are better prepared to design and implement culturally appropriate interventions. (Contains 3 tables and 2 figures.)