Determining the reflections of technology integration applications that are to be performed in our schools is important to light the way of first steps of integration. In this research, the effect of a web-based instruction environment used by 31 different teachers in a high school to school culture is set forth. The school culture is analyzed within the frame of thoughts and relations of teachers and directors and the spread of these applications that are new for the school. Case study methodology has been applied in this study; the qualitative and quantitative data have been assessed together. The qualitative data of the study include the interviews with teachers, in-class observations, interviews with school directors; the quantitative data are obtained from the surveys applied on teachers. In addition to this, the records obtained from web-based instruction environment have been also used for interpreting other data. At the end of the study, positive changes have been detected over the thoughts and relations of teachers and directors. Moreover, during this research, even though there have been some exceptions, spread of web based instruction activities among teachers has taken place in parallel with the general technology diffusion theory. As a result, we may say that thanks to this research, a culture based on the changes on the teachers and directors and school, usage of technology has started to be formed. Within this direction, the directors, teachers who are the important elements of school culture for technology integration have made suggestions for the applications to be applied in school.