The effectiveness of five different enzymes as treatments against Staphylococcus biofilm growth was measured in the presence of antibiotics and alone. Protease was the least effective enzyme in biofilm removal with all antibiotics, and pectinase was the most effective with dicloxacillin and clindamycin. Also, dicloxacillin was the most effective antibiotic. "S. epidermidis" was resistant to ciprofloxacin except in the presence of amylase and pectinase. Overall, there was a statistically significant difference among the treatments on biofilm absorbance (ANOVA; df = 23; F = 2.06; p = 0.009). Because of limited growth in the biofilm assay, no significant difference was found between the treatment groups and the control. However, a zone of inhibition study found that planktonic growth was unaffected by enzymes but inhibited by all antibiotics tested. Results support that enzymes may aid in antibiotic effectiveness against biofilm growth, and more extensive testing is warranted to explore why the impact differs in the biofilm structure. (Contains 3 figures.)