The researchers conducted this study to investigate undergraduate preservice teacher candidates' perceptions regarding variables related to instructor presence in online courses. Participants included 52 undergraduate education students enrolled in 100% online technology integration courses at a doctoral research university in the southeastern United States. The researchers used a mixed-methods design and analyzed quantitative data, collected via an online survey, using descriptive statistics and content analysis. The results of each analysis confirmed the other. The main findings were that timely responses, clear instructions, instructor availability, and course design are important concerns of the participants in this study. (Contains 2 tables.)