This study explored the relationship between spirituality and work values among graduating college seniors at a large public mid-Atlantic Research-Extensive institution. Gender differences in this relationship were also examined. Via an online survey, 429 participants completed Super's (1968) Work Values Inventory and Wheat's (1991) Human Spirituality Scale. The results revealed a low, but statistically significant relationship between spirituality and intrinsic work values (r=0.40). Between spirituality and extrinsic work values, an even lower, but statistically significant relationship existed (r=0.15), with men exhibiting higher degrees of relationship than women. These findings inform the practice of career counselors by raising awareness of gender influences on spirituality and career development. (Contains 3 tables.)