The Web-based e-learning can benefit from the modern multimedia tools combined with network capabilities to overcome traditional education. The objective of this paper is focused on e-training system development to improve performance of theoretical knowledge and providing ample opportunity for practical attainment of manual skills in virtual environment. A conceptual design Web-based medical information system including an e-training methodology and a model of content relations is described. The system helps the practitioners to keep up to date with the latest scientific breakthroughs and state-of-the-art laparoscopy techniques. The developed prototype of Web-based medical information system for laparoscopy e-training is illustrated. (Contains 4 figures.)