This paper presents the findings of a study on the current knowledge and attitudes of pre-service teachers on the use of scenario-based multi-user virtual environments in science education. The 28 participants involved in the study were introduced to "Virtual Singapura," a multi-user virtual environment, and completed an open-ended questionnaire. Data from the questionnaire indicated that gender and current computer game use were likely to affect the perceived benefits of using virtual worlds in a classroom setting. Behavior management was seen as being a constraining factor on a pre-service teacher's willingness to use a virtual world in the future. Overall, the results of the study indicate that pre-service teachers as a result of their use of "Virtual Singapura" are both aware of virtual worlds and have a reasonable understanding of both their potential advantages and disadvantages within a classroom setting. (Contains 3 figures and 5 tables.)