Objectives: The purpose of this study was to assess potential relationships among reproductive health knowledge, preventive health behaviors, perceived severity and risk of breast cancer, cervical cancer, and sexually transmitted infections and selected demographical variables and characteristics related to acculturation among Latina immigrants. Methods: A total of 204 Latinas from a Midwestern city in the United States consented to participate in the study. Health interviews were conducted using semistructured interviews and convenience sampling. Interviews were conducted in the participants' choice of language by trained bilingual administrators at a variety of Latino associated locations. Results: Characteristics associated with acculturation, such as English skill, play a role in preventive health behaviors--in this case, self breast exams and likelihood of getting Pap tests. Perception of risk and severity may be affected by acculturation characteristics such as barriers to access as well as perception of severity which appear to be higher among Latinas with lower education levels. Conclusion: Culturally appropriate efforts to assess the health needs of emerging Latino communities will contribute to preventive health education for women. (Contains 3 tables.)