Purpose: Study 1 developed the Assessment of Attitudes Toward Augmentative and Alternative Communication-2 (AATAAC-2) to assess adolescents' attitudes toward peers who use AAC. Study 2 used the AATAAC-2 to examine influences of familiarity with people with disabilities; type of AAC device; and various combinations of gender of rater, AAC user, and communication partner on adolescents' attitudes. Method: In Study 1, 194 adolescents viewed videotapes depicting adolescents using AAC, then completed AATAAC-2. Study 2 utilized 8 videotapes depicting 4 different gender combinations of AAC user and communication partner as experimental stimuli. Each gender combination was filmed twice: once with a static touch screen device, and once with a dynamic touch screen device. One-hundred thirty-six adolescents were randomly assigned to view 1 of the 8 videos. Participants then completed AATAAC-2. Results: Study 1 demonstrated that AATAAC-2 has adequate psychometric properties. Raters' responses in Study 2 indicated no main effect of device type; girls were more positive than boys; and familiarity with peers with disabilities was associated with more positive attitudes. No 2-way interactions were significant; 3-way interaction of level of familiarity, gender, and type of device used was significant. Conclusions: Familiarity and gender contribute to adolescents' attitudes; type of AAC device combined with these factors to influence attitudes.