The aim of this research is to develop the Mobbing Scale and examine its validity and reliability. The sample of the study consisted of 515 persons from Sakarya and Bursa. In this study, construct validity, internal consistency, test-retest reliability, and item analysis of the scale were examined. As a result of factor analysis for construct validity, four factors have emerged which are named humiliation, discrimination, sexual harassment, and communication barriers. These subscales consist of 23 items and account for the 59.97% of the total variance. The internal consistency reliability coefficients were 0.91 for humiliation, 0.77 for discrimination, 0.79 for sexual harassment, and 0.79 for communication barriers. Findings also demonstrated that item-total correlations ranged from 0.54 to 0.78. Test-retest reliability coefficients were 0.78 and 0.91 for four subscales, respectively. The item-total correlations ranged from 0.54 to 0.78. According to these findings the Mobbing Scale can be regarded as a valid and reliable instrument that could be used in the field of education and psychology.