We examined the association between work-related stress of both spouses and daily fluctuations in their affective states and dyadic closeness. Daily diary data from 169 Israeli dual-earner couples were analyzed using multilevel modeling. The findings indicate that work stress has no direct effect on dyadic closeness but rather is mediated by the spouses' negative mood. Evidence was found for spillover of stress from work to mood at home, as well as negative crossover among couples with higher marital quality, resulting in greater distance on stressful days. Such increased distance may reflect either a deleterious effect of work stress on marital relationships or a protective mechanism used by couples in times of stress.