The purpose of this study was to determine the immediate and long-term effectiveness of a university level conceptually-based health-related (CBHR) fitness course on health-related fitness (HRF) knowledge. Two hundred and seventy-seven students served as subjects. Subjects were grouped according to the following criteria. Group 1 represented students that had never taken and/or were not currently enrolled in the required CBHR course. Group 2 consisted of students currently completing the course. Groups 3 through 6 were grouped according to the number of weeks or years that had passed since they completed the required course. Group 3 represented students currently enrolled in school between 1 and 52 weeks post course completion. Similarly, Groups 4 and 5 represented students currently enrolled either 53-104 or 105-156 weeks post course completion, respectively. Group 6 represented students currently enrolled and who completed the concepts course 157 or more weeks prior to their participation in the study. An 80-item, multiple-choice test was used to determine HRF knowledge. One way ANOVA was used to determine significant differences (F (5, 276) = 49.77, p = 0.000) between groups in HRF knowledge. A least significant difference post hoc analysis test determined mean differences occurred between Group 1 and all other groups and Group 2 and all other groups. No mean differences between Groups 3 through 6 were identified. Results of this study suggest students develop an above average level of HRF knowledge immediately following completion of a university level CBHR fitness course. Additionally, it was concluded students retain and present, for a minimum of four years, significantly higher levels of HRF knowledge than students that have never taken or completed a university level CBHR fitness course. (Contains 3 tables.)