Business incubators play a critical role in economic regeneration through the development and support of new and sustainable enterprises. Many UK incubator projects are funded by the European Commission through the higher education sector. This study compares and contrasts six business incubation case studies and identifies significant criteria for successful operation and ongoing sustainability. A conceptual framework for a "ladder" of business incubation, supporting new businesses through start-up and growth, is proposed as a guide to good practice. The findings clearly identify the need for key partnerships with public-sector and private-sector stakeholders to ensure incubator longevity. (Contains 4 endnotes, 2 tables, and 1 figure.)