Virtual reality (VR) provides several potentials to unlock in today's world. VR is a computer interface that allows users to interact with each other or with the media in a computer-generated three-dimensional space using senses (Piovesan, Passerino, & Pereira, 2012). One of the most important VR environment is Second Life. It provides both for educators and students the opportunity to deviate from the habits, to leave the traditional framework of teaching and learning (Chen, 2016). In our research, we created a virtual learning environment in the Second Life that is called Fornax. The devices placed in the area were intended to provide a thorough introduction to the different parts of the digestive system. In a virtual space, students can easily learn about human organs, as they also can see what they are learning, which can be a huge help in acquiring information (Huang, Liaw, & Lai, 2016). Based on the results, we could state that test results of users who are more familiar with Second Life are significantly better respondents than the beginner users. However, one of the four types of questions that of multiple choices with short answers provided the same result.