Zoonoses, or diseases that can be transferred between humans and animals, such as variant swine influenza, avian influenza A viruses, and coronaviruses, comprise a large percentage of all newly identified and existing infectious diseases (Centers for Disease Control and Prevention, 2017). As a result, there is an ever-growing need for a One Health approach towards preventing and controlling zoonotic diseases. To better manage these public health risks at the human-animal-environment interface, Maryland 4-H and Maryland Department of Health initiated a statewide, multi-agency collaborative partnership. Through steps taken to collectively develop and implement long-term strategies to prevent future variant influenza outbreaks in Maryland, a valuable One Health partnership was developed. As a result, the Healthy Animals - Healthy YOUth program, novel influenza, and other zoonotic disease subject matter content was delivered using multiple approaches, including 1) the addition of new content to an already existing online 4-H Animal Husbandry and Quality Assurance program, 2) distribution of 120 resource kits across two states containing a collection of 24 hands-on lessons, resource documents, and supplemental teaching materials, 3) in-person and virtual train-the-trainer sessions, 4) youth workshops, 5) educational and hands-on activity videos, and 6) standardized fair signage for use at animal exhibits.