Although researchers have used practical procedures for developing professional teacher education, such as reflective practice and action research, the role of learning-oriented assessment (LOA) in building an active learner-centered pre-service teacher education has received scarce scholarly attention. Hence, the purpose of this study is to explore how collective and reflective LOA can enhance EFL pre-service teachers' learner-centered teacher education. For this purpose, 15 Iranian EFL pre-service teachers were selected through a convenience sampling procedure from a teacher-training college in Iran. The study was built on a qualitative intrinsic case study, the data were collected from the participants through observations, self- and peer-assessment practices, portfolios, and semi-structured interviews, and the collected data were inductively analyzed. The results of the inductive thematic analysis indicated that pre-service teachers became active, cooperative, and flexible. Firstly, pre-service teachers concentrated on reflective self-assessment, collaborative peer-assessment, and portfolios. Secondly, pre-service teachers were more self-directed and autonomous by taking more actions, planning their development, making decisions, and learning. Thirdly, pre-service teachers accelerated their positive emotions, such as growth mindset, flexibility, curiosity, and criticality. The findings promise implications for implementing learning-oriented assessment to promote teachers' agency and autonomy in their teaching careers.