Background: Fostering students' interest in careers in Science, Technology, Engineering, and Mathematics (STEM) is one of the main objectives of current education. However, stereotypical beliefs about STEM careers and their difficulty often exist among elementary and high school students, especially in Latin American countries where access to higher education can be complicated or seem impossible in some areas. This study aims to develop effective strategies to combat stigmas by integrating Colombian ancestral pacific traditional knowledge and biochemistry engineering and promoting STEM education in populations with limited access to higher education in Colombia. The goal is to help participants understand the broader application of science in their daily lives and communities. Results: By integrating pacific traditional knowledge with bioprocess experiments through our culturally relevant lab box, we have fostered greater interest in STEM careers among these diverse participants. This methodology effectively bridges the gap between traditional wisdom and modern science, creating a more engaging and relatable STEM learning experience. Conclusions: In conclusion, we have observed a noticeable shift in participants' academic interests, and many have expressed a new enthusiasm for pursuing STEM-related careers. This project provides a highly viable and reproducible model that can be scaled to other cities or countries in Latin America, facilitating students' initial engagement with STEM careers. Additionally, it offers scholarship opportunities for participants, enabling access to quality higher education and promoting gender inclusion in STEM fields.