This paper examines the cultural implications of Gothic-themed lexemes in English and Uzbek, emphasizing their linguistic structures and semiotic meanings as well as educational implications. We have tried to do a comparative linguistic analysis to investigate lexemes from literary texts, dictionaries, and cultural studies, revealing their thematic and cultural significance. The findings indicate similarities and differences in the Gothic lexicon of the two languages, influenced by unique historical and cultural developments. English Gothic lexemes embody themes of autonomy, enigma, and existential dread, whereas their Uzbek equivalents are interwoven with folklore and spiritual elements, highlighting communal experiences and moral dichotomies. These results emphasize the versatility of Gothic aesthetics across countries, providing fresh perspectives on cross-cultural literary and linguistic studies.