The primary objective of this research is to investigate the relationship between organizational culture, job characteristics, and burnout among teachers affiliated with the Phetburi Primary Educational Service Area Office 1. Additionally, we aim to examine the predictive influence of organizational culture and job characteristics on burnout. To achieve these objectives, we conducted a study with a sample group comprising 241 school teachers under the Phetburi Primary Educational Service Area Office 1. Data collection instruments utilized a Likert scale with a 5-level conformity index ranging from 0.60 to 1.00 and a confidence level of 0.959. Statistical analysis methods included frequency distribution, percentages, mean, standard deviation, Pearson correlation coefficient, and multiple regression analysis. The research findings underscore the moderate level of burnout among teachers under the Phetburi Primary Educational Service Area Office 1. Organizational culture and job characteristics correlate negatively with teacher burnout in schools, with statistical significance at the .01 level. Furthermore, these factors significantly influence teacher burnout at the .01 level. Combined, they predict teacher burnout in schools at 58.2%, with organizational culture exhibiting the highest predictive power at 99.6% in regression analysis, while job characteristics have a predictive power of 83.4%.