The purposes of this research were to study the level of participation in the environmental conservation of undergraduate students and to study and compare the factors affecting participation in the environmental conservation of students of different genders and academic years. The sample used in the research were 231 undergraduate students of 1-4 years in the Environmental Education, Faculty of Environment and Resource Studies, Mahasarakham University, from voluntary sampling. The tools used in the research include participation factors in environmental conservation. The statistics used in data analysis were frequency, percentage, mean, standard deviation, and One-Way ANOVA. The results showed that: (1) The level of factors affecting participation in environmental conservation of undergraduate students, classified by aspect, it was found that undergraduate students participated in environmental conservation at a high level, equal to 3.52. When considering each aspect, it was found that all aspects were at a high level, including leadership factors, support factors (cooperation, public sector, government sector, and private sector), personal attributes factors (gender, age, year, domicile), environmental factors (economic, social, environmental cultural aspect), media factors, legal factors, educational factors, and ideological/ inspirational factors, respectively; and (2) There was no difference in students who have different genders and academic years in participation in environmental conservation.