Applying the idea of green chemistry, we extended an esterification teaching experiment with isoamyl alcohol and acetic acid and reused isoamyl acetate in the transesterification reaction with KOH as the catalyst. As a result, isoamyl alcohol was produced and could be reused for the teaching experiment. The simplified experimental method achieved high purity for the product. This esterification reaction does not produce more waste liquid, and because the isoamyl alcohol can be reused, the cost of reagent procurement and waste liquid disposal is reduced. This green experiment offers not only environmental benefits but also an important learning experience for students. It improves their skills and cultivates a consciousness of innovation and environmental protection.