This paper examines the University of Sydney Library's development and piloting of a methodology to survey its Aboriginal and Torres Strait Islander cultural collections, to enhance catalogue metadata and allow culturally sensitive material to be identified and protected. The research falls broadly within the interpretivist epistemology and draws methodologically on Participative Action Research. The pilot survey was conducted using a qualitative, collection-based approach, more specifically Direct Collection Analysis. A broad syntax was used to identify cultural content within the catalogue metadata, and items individually examined to determine the nature of their contents. The findings surface the benefits and shortcomings of the initial methodology. It is recommended future surveys be conducted using more specific terminology checklists and undertaken by a dedicated team that has received care and handling training for cultural materials and is led by an Aboriginal or Torres Strait Islander expert in cultural collections.