The use of virtual museums in cultural transfer is an interdisciplinary approach of significant importance. Virtual museums play a crucial role in cultural transfer by digitally preserving cultural heritage. This article examines the educational role of virtual museums as a key tool in cultural transfer, particularly in the context of representing Ottoman works in virtual museums. Museums, indispensable elements of cultural transfer and education in contemporary society, have gained the opportunity to reach wider audiences by transitioning into the digital realm. In this context, the representation of Ottoman works in virtual museums and its role in cultural transfer are highlighted. Virtual museums hold great educational potential. It is well recognized that the representation of Ottoman works in virtual museums has educational effects such as expanding learning environments, facilitating access to knowledge, and increasing cultural awareness. Therefore, the representation of Ottoman works in virtual museums, especially in the field of education, is addressed. This study evaluates the role of the representation of Ottoman works in virtual museums in cultural transfer and education, thus demonstrating the educational potential of virtual museums. The research illustrates that virtual museums serve as significant tools for the preservation and dissemination of cultural heritage, and the representation of Ottoman works in virtual museums contributes to this process. Using Ferdinand de Saussure's theory of signs, signifier, signified, this study examines the representation and educational role of Ottoman works in virtual museums.