This study aims to determine the need for multitiered diagnostic instruments to identify misconceptions and their causes, as well as multi-representational profiles in chemistry lessons starting from grade 10 in the initial to the final material for grade 12. The data analysis technique used in this research is descriptive, namely descriptive analysis. Systematic and factual facts are investigated by collecting data, processing and analyzing. The instruments used have been validated by expert lecturers and received improvements in the diction of the question sentences. The subjects used in this study were several chemistry teachers in East Java, totalling 67 teachers who were taken by random sampling. Data collection was carried out through questionnaires distributed using the media google form. The results of the analysis found that diagnostic assessments in chemistry subjects were made up to the macroscopic, submicroscopic, and symbolic levels with a percentage of 93%; it is necessary to apply diagnostic assessments to all chemistry learning topics with a percentage of 61%, diagnostic assessments in chemistry subjects must be carried out in a multitiered manner level 5 with a percentage of 79%.