Numerous studies have proven the learning benefits of concept maps in science subjects, particularly for students with low prior knowledge. There is a scarcity of research dedicated to the examination of chemistry courses at the university level, and the findings pertaining to academic performance in that subject exhibit a lack of consistency. This study examined the impact of concept maps on students of a General Chemistry course who had low prior knowledge. The study applied a quasi-experimental design to collect data on two topics: uncertainties of measurements (Topic 1) and acid-base (Topic 2). Fill-in-the-nodes concept maps were developed and served as learning materials. ANCOVA and Johnson-Neyman techniques were used to analyze the scores of concept tests of Topic 1 and Topic 2, respectively. In both Topics 1 and 2, the results showed that the treatment group outperformed the control group. However, the aforementioned finding was limited to the subset of students whose pretest scores were below 30.7 out of a total of 47. From the analysis of the attitude questionnaire, the authors concluded that the students appreciated the usefulness of concept maps. However, they might hesitate to engage in using this new learning tool. The study's findings strengthen the evidence of the learning benefits of concept mapping. Moreover, using concept maps in teaching is feasible because of their low cost and minimally invasive modification to instructional design. The practices for implication of concept mapping are also discussed.