This study aims to explore the impact of perceived transformational leadership on knowledge sharing behavior among university teachers in Anhui Province, China. Using a questionnaire survey method, convenient sampling was conducted among college teachers in Anhui Province. With 326 teachers as the research subjects, SPSS software was used to analyze the relationship between teachers' perception of transformational leadership and knowledge sharing behavior in the context of colleges and universities. Additionally, this study compared the impact of demographic variables such as age, teaching experience, and professional title on teachers' knowledge sharing behavior. The research results indicate that Anhui university teachers' perception of transformational leadership has a significant positive impact on knowledge sharing behavior. Moreover, there are significant differences in teachers' knowledge sharing behavior based on their age, teaching experience, and professional titles.