This exploratory cross-sectional study aimed to identify whether specific sociodemographic and attitudinal characteristics could predict educators' comfort working with sexuality minority people and willingness to take action in their own school or classrooms to help children and sexuality and gender minority parents feel accepted and welcome. The study included 122 early childhood educators working in the northern part of Greece. The School Professionals' Attitudes Towards Homosexuality (SPATH) measure was used to examine comfort and action orientation to work with sexuality and gender minority parents and their children. The study's findings show that educators' sexual prejudice affects the promotion and endorsement of sexuality and gender minority families' rights in school settings. Findings emphasise the need to implement training to contribute to reducing prejudice about specific minorities and facilitate inclusion in school settings.