A child with Down syndrome (DS) is physically characterized by muscle hypotonia, joint instability, and poor motor coordination. Here, we tested whether Dohsa-hou training could improve motor coordination among children with DS, compared to a control condition. Forty children with DS were randomly assigned either to Dohsa-hou training or to a control condition. All participants completed a bimanual coordination test, at the following time points: baseline, seven weeks later at completion of the intervention, and again 4 weeks later at follow-up. Bimanual coordination accuracy and consistency improved from baseline to intervention completion and to follow-up, but only in the Dohsa-hou training, compared to the control group. The findings suggest that among children with DS and compared to a control condition, Dohsa-hou training has the potential to enhance the bimanual coordination, thus contributing to improved motor control of children with DS.