Many studies have found associations between structural characteristics and interaction quality of children in ECEC settings. However, findings are inconsistent and since previous research has been conducted outside Austria, transferability to the Austrian country context is limited. By addressing this gap, the study aims to identify relations between structural characteristics and interaction quality (measured with the Individualized Classroom Assessment Scoring System, inCLASS) of 161 three- and four-year-old children from 55 preschools in Austria. After including control variables, main findings revealed positive relations between adequate equipment and teacher interactions and negative relations between child-staff ratio, proportion of children with immigration background per preschool class, preschool teachers' work experience and peer interactions. Preschool teachers' job security was positively related to task orientation and poorer child-staff ratio corresponded with more conflict interactions. Higher work experience led to fewer conflict interactions. The results indicate that specific structural characteristics are associated with a higher interaction quality in preschools in Austria.