Long-term energy supply constitutes a pressing global issue. As a renewable energy resource, bioenergy can play a significant role in energy sustainability. The present study aimed to investigate the attitudes and perceptions of bioenergy amongst 412 Greek preservice primary teachers utilising a specific tool, the IBPAMS. Results confirmed the factorial validity and reliability of the Greek version of the IBPAMS. It also demonstrated that the energy and bioenergy-related knowledge of preservice teachers is moderate and poor respectively, while critical perceptions are held over the sustainability of forest-based bioenergy production. Overall preservice teacher attitudes toward bioenergy are positive. Statistical analyses showed that gender influenced the responses of the motivational and critical aspects of the IBPAMS-GR. The paper's findings have important implications for teacher preparation programs.