Biology learning in the 21st century makes students understand concepts, including excretory system learning. Selfregulated learning is how students learn independently, with digital literacy skills, which should be maintained as students must control their use of technology and information in learning. This research aimed to analyze the relationship between self-regulated learning with the conceptual understanding of the excretory system and digital literacy with the conceptual understanding of the excretory system. Then also, the relationship between self-regulated learning and digital literacy as a whole with the conceptual understanding of the excretory system in a high school with a post-pandemic learning situation. This research was held for two months, from March until April 2022, in SMAN 6 Jakarta, involving 94 students of class XI Science as samples. A descriptive correlational study was chosen, while questionnaires and tests were used as instruments. The hypothesis test shows a multiple linear regression model in Y=-12.495+0.345X[subscript 1]+0.680X[subscript 2] with the model significance of 0.000 and 0.062 as relationship linearity, then concludes that there is a positive relationship which is significant and linear between self-regulated learning and digital literacy simultaneously with excretory system conceptual understanding.