The COVID-19 pandemic has caused an increase in the use of e-learning software. From the perspective of the decision-makers (school/university administration), it is crucial to understand what characteristics of the software are perceived by the users (teachers) as necessary for a task (e-learning). A popular method of determining these characteristics is a technology acceptance model. In this paper, the authors aim to understand the technology acceptance of Microsoft (MS) Teams by applying the Unified Theory of Acceptance and Use of Technology (UTAUT) model. The authors also analyze acceptance in two distinctive groups of teachers: schoolteachers and academic teachers, to better understand differences in e-learning software acceptance and implementation. The results show that MS Teams is a well-accepted software and the users' intention to use MS Teams in e-learning is the same regardless of the teacher's group. There are significant differences in the perception of software characteristics between different groups. The authors analyze the potential causes of these differences and suggest practical implications for improving the quality of e-learning. The intent is not to validate UTAUT as a general technology acceptance model but to understand its practical importance in explaining use behavior in e-learning.