This study aimed to investigate the effect of part and whole practice on learning basketball lay-up shot skill in young and adolescent male students. Participants were randomly divided into four groups of part and whole practice, namely, part practice-young, whole practice-young, part practice-adolescent, and whole practice-adolescent. After a pretest in basketball lay-up shot test, the training protocol was held for 5 weeks (three sessions per week). After the last training session, the posttest was taken, and 1 week after the posttest, a retention test was performed. The results in the acquisition stage showed a significant difference between the four groups (p = 0.03). The post hoc test results showed that there was no significant difference between the part and whole practice groups. Also, in the retention phase, there was no significant difference between the groups. However, the part practice-young group performed better than the whole practice-young group, while the whole practice-adolescent group performed better than the part practice-adolescent. According to the research findings, it can be concluded that age is probably not an effective factor in the effectiveness of part and whole practice in learning basketball lay-up shot. However, more research with more practice trials is needed in this regard.