We evaluate the impact of a low-stakes easy-to-implement course-level intervention, Scientist Spotlight assignments, which feature personal and professional stories of diverse scientists. This work extends previous studies by examining whether shifts in relatability differ across student identities, particularly students who identify as first-generation students, a population that has not been the focus of previous investigations of this intervention. Using paired pre- and postcourse data from four implementations in an introductory biology course, we report a significant, positive shift in undergraduate students' self-reported ability to relate to scientists, and concomitant shifts in how students describe scientists after completing four or six Scientist Spotlight assignments. Importantly, our data demonstrate a disproportionate, positive shift for first-generation college students and for students who identify as female, a novel contribution to the body of literature investigating the Scientist Spotlight intervention. This study, along with previous reports of similar shifts in varying institutional contexts across different populations of learners, provides a strong argument that instructors interested in diversifying their course content to include representations of diverse scientists to enhance students' ability to identify a range of "types of people" who do science can do so successfully through incorporation of a small number of Spotlight assignments.