Differentiated instruction (DI) is an inclusive method of instruction by which teachers provide multiple possibilities for learning based on students' backgrounds, readiness, interests, and profiles. Acknowledging student diversity in Canadian classrooms, this study explores STEM teacher candidates' (TCs') preparation to implement DI in a STEM curriculum and pedagogy course in a teacher education program. The course is enriched with DI resources and training focused on equity, diversity, and inclusion (EDI). The course efficacy in enhancing TCs' implementation of DI is explored through the following research questions: (1) What is the impact of the course on TCs' implementation of DI, (2) How do TCs develop curricula to be inclusive of DI strategies, and (3) What successes and challenges do TCs encounter when developing DI-focused curricula? The study adopts a mixed-method approach, in which data sources include pre-post questionnaires and semi-structured interviews. Participants are 19 TCs enrolled in the second year of the teacher education program at a Canadian university. Findings suggest that the course empowered TCs to integrate DI principles and strategies in their coursework. This success reiterates the importance of opportunities aimed at enhancing teachers' preparation to incorporate DI in their practices. The findings call for adopting similar approaches in pre-service and in-service teachers' training to ensure that DI principles and strategies are deeply rooted in teachers' practices. The study informs teacher educators about integrating EDI in teacher education programs' curriculum and overall planning.