Effective use of information resources by lecturers is one of the determinants of university education outcomes. To function optimally, lecturers utilise digitised information resources in their duties. University libraries are saddled with the responsibility of organising and providing lecturers' needed information resources. This study, therefore, investigated the influence of digitised library information resources availability on the use of information resources by lecturers in six federal universities in Southern Nigeria. Instruments used for data collection were the DLIRA (r = 0.73) and UIR (r = 0.64) scales. Data were analysed using descriptive statistics, the Pearson product moment correlation, and multiple regressions at 0.05 level of significance. The results showed that digitised library information resources availability on the use of information resources influenced the use of information resources by lecturers in the federal universities in Southern Nigeria. Thus, there is a need for the management of university libraries to intensify efforts at converting most of the paper-based resources to digitised formats for increased use of library resources by lecturers.