This study aimed to develop the "Students' Perception of Instructional Feedback Scale" (SPIFS) determining a framework related to the perception of instructional feedback by students. The sequential exploratory mixed method was used in the study. The study was conducted during the instructional design course offered to sophomores in the Department of Computer Education and Instructional Technology at two different universities. Accordingly, firstly a scale consisting of 31 items with Likert-type responses was prepared based on the literature review. Validity and reliability analyses of the scale were completed with a total of 231 participants. After necessary steps were applied in exploratory factor analysis (EFA, n=100), a structure with three factors and 19 items was established. The internal consistency analysis (Cronbach's alpha), which was applied to the factors obtained and the whole scale, showed the scale to be reliable (whole scale [alpha]=0.85, 1st factor (mastery, 8 items) [alpha]=0.92, 2nd factor (positive affect, 6 items) [alpha]=0.90, and 3rd factor (negative affect, 5 items) [alpha]=0.96). Confirmatory factor analysis (CFA) was performed (n=131). The structure established through EFA was tested via CFA. The results indicated that the developed structure had acceptable fit (RMSEA=0.08, CFI=0.91, and RMR=0.03).