Nearly half of all college students and the majority of college students of color begin their studies at 2-year colleges. The educational quality that these students experience will affect future success, but little research to date has focused on the professional development (PD) of their instructors. We offer an exploratory study on PD needs and preferences of ten 2-year college biology instructors who have experience with evidence-based instructional practices. Using a literature review and interview data, we address four research questions. We contextualize the interview results by describing interviewee teaching styles and their teaching and inclusion strategies, drawing on categorizations from education research literatures in and beyond biology. We then summarize interviewee experiences, preferences, and recommendations for PD. Most interviewees preferred PD that could be readily applied to their courses and included follow-up community support. While our purposive sample is limited, we note high levels of interest in PD supporting inclusive pedagogy and non-biology learning goals, such as study skills, metacognition, and quantitative skills. We describe implications for inclusive design of biology instructor PD.