The purpose of this research was to organize a cross-cultural adaptation study and analyze the reproducibility and test-retest reliability of the Infant Sensory Profile 2 (ISP2Br) to Brazilian babies. It was hypothesized that the instrument would be validated for use with Brazilian babies. The English language version of the profile was translated and culturally adapted into Brazilian Portuguese, then administered to a group of 135 caregivers of infant from birth to 06 months and then re-administered to a portion of the sample (35 caregivers) for 7 to 14 days for test-retest reliability. Participants were recruited from an outpatient pediatric clinic of a federal university in Brazil and at two pediatric research laboratories. The internal consistency and test-retest reliability was analyzed using the Cronbach's alpha and kappa coefficient, respectively. As it is a norm-referenced standardized assessment, the cutoff scores used were 1.0 and 2.0 standard deviations above and below the mean for each group of items established as the preliminary cutoff scores for the Brazilian children. Findings showed that cultural adaptation led to adjustments in some terms used in the ISP2Br and it showed good internal consistency (>0.70) relating to the total scale; however, when it was analyzed for sensory areas, all alpha values were <0.70. Regarding the test-retest, the values fell into the category of near-perfect agreement (0.81 < k < 1.00). The ISP2Br was found to be valid and reliable and allows occupational therapists to identify differences in sensory patterns in Brazilian babies compared to the original standardization sample, responding differently from would expect.