Nowadays, we talk about the use of gamification in education, an active methodology that consists of the use of mechanics, design or game structures in class. When this type of methodology is used, the effort is rewarded, and it is used as a motivating tool in class. However, there is no valid or well-structured instrument to measure gamification properly in education. Aim of this study is to develop and validate an instrument to measure the experience of gamification in educational contexts (EGAMEDU) as a valuable tool of diagnosis for the teaching staff to guide their teaching practices to include this methodology. A sample of 401 participants is used for the validation of the questionnaire related to education and gamified experiences. The results demonstrate good validity indices and a factorial structure according to the one proposed in the theory.