Objectives: The purpose of the current study was to examine the effects of using an activity workstation on the physiological stress response as measured by heart rate variability while completing cognitively demanding tasks. Participants: Eleven college students (6 females; age: 19.4 ± 0.9 years) participated in the study. Methods: The participants completed three psychologically stressful cognitive tasks while seated at a traditional desk and while using an activity workstation. Heart rate variability was recorded and analyzed with power spectrum density and time-domain analysis. Results: Using activity workstations while completing stressful cognitive tasks did not negatively affect task performance. There was, however, a reduction in low frequency heart rate variability but no change in cardiac sympathovagal balance. Conclusion: The results indicate that using activity workstations while completing difficult tasks reduces sympathetic reactivity to stress in college students. This suggests that using activity workstations could provide a coping mechanism for stress.