ERIC Number: EJ1327787
Record Type: Journal
Publication Date: 2022-Feb
Pages: 6
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: N/A
Available Date: N/A
Teaching the Use of Micropipettes through Direct Visualization of Data: An Experiment Focusing on Technique, Skill, and Accuracy
Goach, Audra L.; Croslow, Seth; McLaughlin, Kyle; Distin, Steve
Journal of Chemical Education, v99 n2 p886-891 Feb 2022
The correct use of micropipettes has been investigated in an undergraduate, introductory lab experiment intended for chemistry and biology majors. This lab experiment involves the use of micropipettes for accurately mixing, transferring, and applying a specific amount of dye to a cotton bandana. The data resulting from this experiment is a color wheel that displays the amount of dye transferred based on the volume of the micropipette set by the student. Student learning outcomes for this experiment include the ability to choose the correct micropipette for the volume needed, set the correct volume on a micropipette, properly draw up solutions, and use proper dispensing technique. The development of experimental foresight and improvement in students' planning was an unintentional learning outcome. A prelab survey assessing students' confidence in accurately using micropipettes shows that a majority of first-year students had not used micropipettes in high school (85%) but did in their first-semester science course (60%) before taking this course. The majority of those students have confidence in their ability to choose the correct micropipette, draw up the liquid, and dispense it properly. There was less confidence in setting the correct volume on the micropipette. The postlab results show that some students still had issues in setting volumes on micropipettes correctly and in dispensing the liquid. Additionally, the postsurvey suggests that setting the correct volume on the micropipette was the most learned skill from performing the lab. Overall, after performing the lab exercise, 92% of the students were confident in their ability to choose the correct micropipette and load and dispense liquid properly compared to 62% before performing the exercise.
Descriptors: College Science, Laboratory Equipment, Undergraduate Students, Science Instruction, Laboratory Experiments, Chemistry, Biology, Laboratory Procedures, Visual Aids, Accuracy, College Freshmen, Self Esteem, Equipment Utilization, Instructional Effectiveness
Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A
Author Affiliations: N/A