Purpose: This research aimed to: (1) measure out the validity level of the developed Student Worksheets, (2) explain the students' responses and learning motivation in using Higher Order Thinking Skills (HOTS)-based worksheets, and (3) find the effect of the HOTS-based worksheet on learning outcomes. Methodology: The study employed the Research and Development (R&D) approach with the following steps: (1) preliminary study, (2) development, and (3) implementation. The population was all second-semester students (Classes A, B, and C) in 2020/2021. Samples were 30 students of the Informatics Engineering Study Program at the Universitas Muhammadiyah Sorong. The data was collected from learning outcomes, validated worksheets, and student response and learning motivation instruments. Subsequently, the data were analyzed using learning outcomes data, expert validation, and regression analysis. Findings: The results showed that the design of student worksheets could effectively improve students' high order thinking skills, as indicated from their learning results in the good category. On the other hand, the design can also motivate students and activate their responses following the lesson. Implications to Research and Practice: HOTS-based worksheets encouraged students to understand the material and stimulate their thinking-frame to the maximum. Besides, the worksheet can motivate students to learn to understand the learning materials more easily.