The study investigated the perception of learners and lecturers on E-learning mode of Education and the availability as well as utilization of facilities for teaching and learning at the Distance Learning Institute (DLI), University of Lagos. Guiding the study are three research questions. The study population consist of all DLI Science education learners and Lecturers. A sample of two hundred and sixteen learners and sixty lecturers (Core and Adjunct) of the Science Education Department, Distance Learning Institute were involved in the study. Detailed researcher-designed questionnaire was the instrument used for data collection. Analysis of data collected was done using frequency counts and mean. Results showed that E-learning facilities such as laptops are inadequate especially on the part of the core lecturers of the institute. Other findings also showed that E-learning facilities that are adequate and available are computers for computer-based test (Examinations), E-library, Internet access while in the institute's premises and Interactive boards in classrooms. Based on these findings, there were recommendations to encourage the use of E-learning facilities for learners as well as lecturers to foster teaching and learning. Hence, there should be regular in-house training for lecturers. Government should provide the required 21st Century E-learning technologies in universities.