Purpose: Simulated competition as a training tool has a relevant role in enhancement of exercise intensity, motivation and physical enjoyment. Including a competitive component into the agility training could represent another way to improve agility performance significantly more. The aim of this study was to assess the effect of agility training performed in the form of competitive exercising on agility performance. Methods: Twenty-two athletes were randomly assigned to an experimental or a control group. Both groups completed the same eight-week agility training, but only the experimental group performed exercises in the form of head-to-head competition. Prior to and after the training, agility time in the Agility Dual test (performed in pairs in the form of head-to-head competition) and Agility Single test (performed individually) was measured. In addition, simple and two-choice reaction time and speed of step initiation were also assessed. Results: A significant group × time interaction effect was observed for agility time in the Agility Dual test only (p = 0.002). This effect occurred in favor of experimental group (18%, p < 0.01). No further significant interactions were detected. Conclusions: Competitive agility training led to significant improvement of agility time under competitive conditions only. This training does not contribute to more pronounced improvements of other reaction and speed abilities.