This study investigated the application of web 2.0 tools for the provision of library services for teaching, learning and research in Polytechnics. The researchers employed a descriptive survey method and a questionnaire was used as instrument for data collection. The population of the study consisted of ninety one (91) library staff in polytechnic libraries in Edo and Rivers states. However, eighty four (84) library staff was found in their respective offices during the period of this study and were subsequently selected as the sample for the study using convenience sampling techniques. The data obtained from the copies of questionnaires retrieved from respondents were analyzed using frequency counts and percentages and mean to answer the research questions. Any item with a mean score of 2.5 and above was considered as an acceptable standard for judgment/ decision making in this study. The results indicated that social networking sites and instant messaging are the most used Web 2.0 applications utilized by the library staff. It was discovered in the study that the library staff in the polytechnic libraries in Edo and Rivers states did not use web 2.0 applications for the provision of library services to users rather for personal purpose to dissemination of information, for communication and for entertainment. The study therefore recommended that government and polytechnic management should be committed to library development by providing the necessary infrastructure and facilities to enable polytechnic libraries render 21st century services to their patrons and the entire polytechnic community.