The purpose of this study was to investigate the relationships between career adaptabilities, career decision regret and burnout of teachers. The participants of the study were 269 teachers (151 females and 118 males) who worked in the primary and secondary education institutions of a city located in the Eastern Mediterranean region in Turkey and agreed to participate in the study. The study used Career Decision Regret Scale, Career Adapt-abilities Scale-Short Form and The Burnout Measure-Short Version were used as data collection tools. SPSS package program and the Process Macro 3.5 software were utilized for the analysis of the data. Pearson correlation analysis was utilized to investigate the relationships between career adaptability, career decision regret, and burnout. Process Macro software developed by Hayes (2018) based on linear regression analysis was utilized to test the power of career adaptability and career decision regret on burnout as well as the moderating role of career decision regret on the relationship between career adaptability and burnout. The results of the study showed that career adaptability and career decision regret predicted burnout significantly. Another finding of the study is that career decision regret played a moderating role in the relationship between the teachers' career adaptability and burnout. Findings of the research were discussed and suggestions were made.