In the academic year of 2019, the designed personalized learning and assessment was applied to the fourth-year pharmacy students who registered for the Pharmacognosy Laboratory in the Faculty of Pharmacy, Silpakorn University. We allowed all students to do the experiment as they preferred. We created a personalized assessment that allowed the students to select the method of assessment by themselves. The evaluations of this personalized learning and assessment were done by using the online questionnaires combined with the students' abilities shown in their reports, their results of the examination, and their contributions to the online reference, KUIHerbRx2017. The results showed that the students performed better with this personalized learning and assessment. The students could select the learning styles and the assessment method which suited them best. The personalized learning made the students feel more satisfied, less stressed, and achieved higher scores.